Microbial contamination of foods can currently only be detected using complex analytical methods in a laboratory. And it often takes days before a final result is obtained 鈥 valuable time during which the undetected contamination could do extensive damage. The research project 鈥淧hoTox 鈥 Single photon detector for on-site detection of bacterial toxins in food using chemiluminescence immunoassays鈥 with the participation of R-Biopharm aims to change that. The outcome should be a rapid, handy, and reliable point-of-care testing.

This project combines immunological toxin detection with a photon detection method. After binding to toxins, modified antibodies generate a light signal. The compact analysis platform is to take advantage of this fact, register single light particles with sensitive photo detectors, and show contamination with the respective toxin on a display.

Six partners are cooperating in the project funded by Germany鈥檚 Federal Ministry of Education and Research (BMBF) with around 2.6 million euros. The partners combine their know-how in the development of immunological detection methods and photon measurement procedures with experience in the design of reaction cartridges and the manufacture of compact detection devices. At R-Biopharm, we are involved in developing the immunological detection method under the direction of Dr. Tobias Wittwer and are providing the necessary antibodies and toxins to be detected.

The other project partners:

  • BYTEC Medizintechnik GmbH 鈥 project coordination and development of the analysis platform
  • Fraunhofer Institute for Microengineering and Microsystems IMM 鈥 development of the reaction cartridge for sample preparation
  • Fraunhofer Institute for Microelectronic Circuits and Systems IMS 鈥 development of the photon detection method
  • Justus Liebig University Gie脽en 鈥 provision of sample material and system testing
  • Ernst Rittinghaus GmbH 鈥 plastics manufacturer, involved in the development of the reaction cartridge and later production
  • Milchpr眉fring Bayern (Bavarian Association for Raw Milk Testing) and Milchindustrieverband (the Association of the German Dairy Industry) 鈥 associated partners that will test the system at the end of the project